Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand
The green robusta coffee bean extract exhibited good antioxidant activity and anti-inflammatory activity. It contained caffeine and chlorogenic acid as major compounds. The aims of this study were to evaluate skin permeation of green robusta coffee bean extract-loaded nanostructured lipid carriers incorporated into the cream (GB-NLCs cream) and study the effectiveness of the formulation in human volunteers. The skin permeation study was tested by Franz diffusion cell using stillborn piglet skin compared between GB-NLCs cream and extract in the cream. The results illustrated that the percentage accumulation amount of chlorogenic acid and caffeine released from GB-NLCs cream was higher than the extract in the cream. The cumulative amount of chlorogenic acid and caffeine in stratum corneum and viable epidermis and dermis from GB-NLCs cream were also higher than the extract in cream due to small particle size, film former, and occlusive effect. The effectiveness of the GB-NLCs cream was evaluated in 15 volunteers in terms of increasing skin moisture and decreasing melanin by Corneometer® and Mexameter®. After using the formulation for 4 weeks, the GB-NLCs cream significantly (p<0.05) increased skin moisture and reduced melanin content when compared with the initial. In addition, skin irritation did not observe in all volunteers after application. Therefore, green coffee bean extract-loaded NLCs in cream was able to improve skin permeation and aesthetic effect on the skin. It has the potential to use it as a commercial skincare product.
